The Synthetic Chemical Biology Core strives to provide comprehensive synthetic chemistry capabilities to investigators under one roof. The synthetic expertise of the core includes, but is not limited to, novel and commercially unavailable small molecules, fluorescent molecules and peptides. The core assists in identifying hits for medicinal chemistry optimization in infectious disease targets and provides synthesis capabilities for structure activity studies of said hits. The core staff will work with investigators to design and synthesis novel molecular probes to facilitate their research. SCB core additionally provides access to the model organism Danio rerio (zebrafish), and allows investigators to image embryonic and adult zebrafish treated with molecular probes for phenotypic drug discovery and other projects. SCB core encompasses the Purification and Analysis Laboratory (PAL) that provides purification, analysis and quality control of compounds via HPLC-MS. The core utilizes automated mass directed fractionation for purification in both reversed and normal phases (including chiral separations), and also provides relative purity analysis by UPLC coupled to a high-resolution mass spectrometer for structure confirmation.
The SCB core is a combination of core laboratories from two NIH-funded COBRE Centers at the University of Kansas: the Center for Chemical Biology of Infectious Disease (CBID) and the Center for Molecular Analysis of Disease Pathways (CMADP).